Lamin B1 Antikörper

Produktnummer ABIN335391

Dieser Anti-Lamin B1 Antikörper ist ein Maus Monoklonal Antikörper zur Detektion von Lamin B1 in WB, ICC und FACS. Geeignet für Human, Maus, Ratte, Rind (Kuh), Kaninchen, Hund, Schaf und Zebrafisch (Danio rerio). Dieses Primary Antibody wurde in 9+ Publikationen zitiert.

Kurzübersicht für Lamin B1 Antikörper (ABIN335391)

Target: Lamin B1 (LMNB1)

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Kaninchen, Hund, Schaf, Zebrafisch (Danio rerio)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Lamin B1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Klon: 119D5-F1

Produktdetails anti-Lamin B1 Antikörper

Spezifität: Human, rat, mouse, bovine, rabbit, dog, sheep.

Aufreinigung: Purified

Immunogen: 119D5-F1 is a mouse monoclonal IgG1/kappa antibody derived by fusion of P3/X63.Ag8.653 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with purified rat liver lamins.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: 119D5-F1 reacts with an epitope located C-terminal of residue 231 in lamin B1. 119D5-F1 is suitable for immunocytochemistry, immunoblotting, ELISA and flow cytometry. Optimal antibody dilution should be determined by titration, recommended range is 1:100 - 1:200 for flow cytometry, and for immunocytochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Referenzen für anti-Lamin B1 Antikörper (ABIN335391)

Broers, Ramaekers, Bonne, Yaou, Hutchison: "Nuclear lamins: laminopathies and their role in premature ageing." in: Physiological reviews, Vol. 86, Issue 3, pp. 967-1008, (2006) (PubMed).

Broers, Bronnenberg, Kuijpers, Schutte, Hutchison, Ramaekers: "Partial cleavage of A-type lamins concurs with their total disintegration from the nuclear lamina during apoptosis." in: European journal of cell biology, Vol. 81, Issue 12, pp. 677-91, (2003) (PubMed).

Neri, Raymond, Giordano, Capitani, Martelli: "Lamin A is part of the internal nucleoskeleton of human erythroleukemia cells." in: Journal of cellular physiology, Vol. 178, Issue 3, pp. 284-95, (1999) (PubMed).

Pugh, Coates, Lane, Raymond, Quinlan: "Distinct nuclear assembly pathways for lamins A and C lead to their increase during quiescence in Swiss 3T3 cells." in: Journal of cell science, Vol. 110 ( Pt 19), pp. 2483-93, (1997) (PubMed).

Broers, Machiels, Kuijpers, Smedts, van den Kieboom, Raymond, Ramaekers: "A- and B-type lamins are differentially expressed in normal human tissues." in: Histochemistry and cell biology, Vol. 107, Issue 6, pp. 505-17, (1997) (PubMed).

Jansen, Machiels, Hopman, Broers, Bot, Arends, Ramaekers, Schouten: "Comparison of A and B-type lamin expression in reactive lymph nodes and nodular sclerosing Hodgkin's disease." in: Histopathology, Vol. 31, Issue 4, pp. 304-12, (1997) (PubMed).

Machiels, Ramaekers, Kuijpers, Groenewoud, Oosterhuis, Looijenga: "Nuclear lamin expression in normal testis and testicular germ cell tumours of adolescents and adults." in: The Journal of pathology, Vol. 182, Issue 2, pp. 197-204, (1997) (PubMed).

Machiels, Broers, Raymond, de Ley, Kuijpers, Caberg, Ramaekers: "Abnormal A-type lamin organization in a human lung carcinoma cell line." in: European journal of cell biology, Vol. 67, Issue 4, pp. 328-35, (1996) (PubMed).

Weaver, Carson, Walker, Chaly, Lach, Raymond, Brown, Sikorska: "Degradation of nuclear matrix and DNA cleavage in apoptotic thymocytes." in: Journal of cell science, Vol. 109 ( Pt 1), pp. 45-56, (1996) (PubMed).

Details zu Lamin B1

Target: Lamin B1 (LMNB1)

Andere Bezeichnung: Lamin B1

Hintergrund: Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2.

Pathways: Apoptose, Caspase Kaskade in der Apoptose